2× Phanta Max Master Mix is a ready-to-use high-fidelity PCR reagent optimized for fast and accurate DNA amplification. It includes Phanta Max DNA Polymerase, dNTPs, and a reaction buffer that supports robust performance across GC-rich, long-template, and complex DNA samples. This mix is suitable for standard PCR, high-fidelity amplification, cloning, genotyping, and mutagenesis.
✅ Key Features
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High Fidelity: Up to 50× higher accuracy than conventional Taq polymerase
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Speed: Amplifies 1–10 kb DNA fragments in seconds per kilobase
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Robust Performance: Reliable results on GC-rich and long templates
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Simple Workflow: Just add primers and DNA templates
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Direct Gel Loading: Contains tracking dyes for direct electrophoresis
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Wide Compatibility: Ideal for plasmid, genomic DNA, and cDNA templates
🔬 Applications
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Routine PCR amplification
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Gene cloning
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Site-directed mutagenesis
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Colony PCR
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High-throughput genotyping
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Next-generation sequencing (NGS) preparation
🧪 Technical Details
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Polymerase Type: High-fidelity DNA polymerase with proofreading activity
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Extension Rate: 15–30 seconds/kb under optimized conditions
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Fragment Length: Efficient for amplicons up to 20 kb (genomic)
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Storage: Stable at -20°C, with anti-freezing buffer
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Concentration: 2× premixed format
🧫 Performance Data
In benchmarking tests, Phanta Max Master Mix demonstrates equal or better performance than standard high-fidelity enzymes. The product maintains high specificity and low background noise, even with difficult templates.
📦 Packaging Options
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1 mL (for ~100 reactions)
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5 × 1 mL
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15 × 1 mL
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Custom bulk options available
